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anti–cd137 pe (17b5) (Thermo Fisher)

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Thermo Fisher anti–cd137 pe (17b5)
Anti–Cd137 Pe (17b5), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti–cd137 pe (17b5)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews

anti–cd137 pe (17b5) - by Bioz Stars, 2026-03

90/100 stars

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(A) Bone marrow cells were analyzed immediately after isolation (day 0) for cell surface marker expression by flow cytometry, or 6×10 6 bone marrow cells were cultured in T25 flasks coated with 1 ml of 10 µg/ml of Fc or <t>CD137-Fc</t> protein and analyzed on days 7 and 14. Black line: Isotype. Gray, filled curve: Marker-specific antibody. (B) Depicted are absolute numbers of live cells (left column) and percentages (right column) of data in (A) for indicated subpopulations at days 0, 7 and 14. (C) Fresh murine bone marrow cells, or cells which were cultured on plates coated with 10 µg/ml CD137-Fc protein for 7 days, smeared on glass slides, fixed with citrate-acetone-formaldehyde solution, and stained for non-specific esterase (black, macrophages) and for specific esterase (purple, granulocytes). Slides were counterstained with hematoxylin and mounted with cover slips. Magnification: ×630. Scale bar: 25 µm. These data are representative of three independent experiments with similar results.

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Journal: PLoS ONE

Article Title: Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

doi: 10.1371/journal.pone.0015565

Figure Lengend Snippet: (A) Bone marrow cells were analyzed immediately after isolation (day 0) for cell surface marker expression by flow cytometry, or 6×10 6 bone marrow cells were cultured in T25 flasks coated with 1 ml of 10 µg/ml of Fc or CD137-Fc protein and analyzed on days 7 and 14. Black line: Isotype. Gray, filled curve: Marker-specific antibody. (B) Depicted are absolute numbers of live cells (left column) and percentages (right column) of data in (A) for indicated subpopulations at days 0, 7 and 14. (C) Fresh murine bone marrow cells, or cells which were cultured on plates coated with 10 µg/ml CD137-Fc protein for 7 days, smeared on glass slides, fixed with citrate-acetone-formaldehyde solution, and stained for non-specific esterase (black, macrophages) and for specific esterase (purple, granulocytes). Slides were counterstained with hematoxylin and mounted with cover slips. Magnification: ×630. Scale bar: 25 µm. These data are representative of three independent experiments with similar results.

Article Snippet: FITC-conjugated anti-mouse Ly-6G (clone 1A8), PE-conjugated anti-mouse Gr-1 (clone RB6-8C5), CD11b (clone M1/70), CD14 (clone Sa2-8), F4/80 (clone BM8), CD137 ligand (clone TKS-1), APC-conjugated anti-mouse CD115 (clone AFS98), Pacific blue-conjugated anti-mouse CD34 (clone RAM34), PerCP-Cy5.5-conjugated anti-mouse CD16/32 (clone 93) antibodies and respective isotype controls (rat IgG2a, rat IgG2b), and PE-conjugated hamster anti-mouse CD137 (clone 17B5) and its isotype control (Golden Syrian hamster IgG) were purchased from eBioscience (San Diego, CA).

Techniques: Isolation, Marker, Expressing, Flow Cytometry, Cell Culture, Staining

CD11b + cells were enriched from bone marrow by MACS, and labeled with CD11b-PE and Ly6G-FITC Ab. 3×10 5 FACS-sorted CD11b + , Ly6G + cells at a density of 6×10 5 cells/ml were cultured on plates coated with 10 µg/ml Fc or CD137-Fc protein for 24 h. Cells were stained with Annexin V-PE and 7-AAD and analyzed by flow cytometry with compensation of FITC channel. PBS: Medium control. This figure is a representative of three independent experiments.

Journal: PLoS ONE

Article Title: Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

doi: 10.1371/journal.pone.0015565

Figure Lengend Snippet: CD11b + cells were enriched from bone marrow by MACS, and labeled with CD11b-PE and Ly6G-FITC Ab. 3×10 5 FACS-sorted CD11b + , Ly6G + cells at a density of 6×10 5 cells/ml were cultured on plates coated with 10 µg/ml Fc or CD137-Fc protein for 24 h. Cells were stained with Annexin V-PE and 7-AAD and analyzed by flow cytometry with compensation of FITC channel. PBS: Medium control. This figure is a representative of three independent experiments.

Techniques: Labeling, Cell Culture, Staining, Flow Cytometry, Control

Murine bone marrow cells at a density of 10 6 cells/ml were cultured for 7 days with respective treatment. 10 µg/ml of Fc or CD137-Fc protein were pre-coated on the plates. (A) Proliferation assay: 1 ng/ml of G-CSF was added daily to indicated wells. Cells (10 5 /condition) were labeled for the last 24 h with 0.5 µCi 3 H-thymidine, and the rate of proliferation was determined with a scintillation counter (Packard, Meriden, CT). Depicted are means ± standard deviations of triplicate measurements. (B) Cell count: 10 ng/ml of G-CSF was added daily to indicated wells. Cells (2×10 6 /condition) were harvested on day 7, and cell count was determined by trypan blue staining of 4 representative microscopic fields. Depicted are means ± standard errors of seven independent experiments. * p<0.05; ** p<0.01.

Journal: PLoS ONE

Article Title: Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

doi: 10.1371/journal.pone.0015565

Figure Lengend Snippet: Murine bone marrow cells at a density of 10 6 cells/ml were cultured for 7 days with respective treatment. 10 µg/ml of Fc or CD137-Fc protein were pre-coated on the plates. (A) Proliferation assay: 1 ng/ml of G-CSF was added daily to indicated wells. Cells (10 5 /condition) were labeled for the last 24 h with 0.5 µCi 3 H-thymidine, and the rate of proliferation was determined with a scintillation counter (Packard, Meriden, CT). Depicted are means ± standard deviations of triplicate measurements. (B) Cell count: 10 ng/ml of G-CSF was added daily to indicated wells. Cells (2×10 6 /condition) were harvested on day 7, and cell count was determined by trypan blue staining of 4 representative microscopic fields. Depicted are means ± standard errors of seven independent experiments. * p<0.05; ** p<0.01.

Techniques: Cell Culture, Proliferation Assay, Labeling, Cell Counting, Staining

2×10 6 murine bone marrow cells at a density of 10 6 cells/ml were cultured for 7 days on plates that had been coated with 10 µg/ml of Fc or CD137-Fc protein. Where indicated, G-CSF was added daily at 10 ng/ml or other stated concentrations. The percentages of Gr-1 + cells (A) and CD14 + , F4/80 + cells (B), as well as absolute cell numbers (E), determined by flow cytometry on day 7, are depicted as means ± standard errors of 7 independent experiments. Dose responses of the combination of G-CSF and CD137 protein: Cells were treated with a fixed concentration of CD137-Fc at 10 µg/ml and varying the concentration of G-CSF at 0, 1, 10, or 100 ng/ml (C); or a fixed concentration of G-CSF at 10 ng/ml and varying the concentration of CD137-Fc at 0, 5, 10, or 20 µg/ml (D), and flow cytometry was performed for Gr-1, CD14 and F4/80 on day 7. These data are representative of two experiments with similar results. Statistical significance was determined by two tailed, paired Student's t-test. * p<0.05; ** p<0.01; n.s. not significant.

Journal: PLoS ONE

Article Title: Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

doi: 10.1371/journal.pone.0015565

Figure Lengend Snippet: 2×10 6 murine bone marrow cells at a density of 10 6 cells/ml were cultured for 7 days on plates that had been coated with 10 µg/ml of Fc or CD137-Fc protein. Where indicated, G-CSF was added daily at 10 ng/ml or other stated concentrations. The percentages of Gr-1 + cells (A) and CD14 + , F4/80 + cells (B), as well as absolute cell numbers (E), determined by flow cytometry on day 7, are depicted as means ± standard errors of 7 independent experiments. Dose responses of the combination of G-CSF and CD137 protein: Cells were treated with a fixed concentration of CD137-Fc at 10 µg/ml and varying the concentration of G-CSF at 0, 1, 10, or 100 ng/ml (C); or a fixed concentration of G-CSF at 10 ng/ml and varying the concentration of CD137-Fc at 0, 5, 10, or 20 µg/ml (D), and flow cytometry was performed for Gr-1, CD14 and F4/80 on day 7. These data are representative of two experiments with similar results. Statistical significance was determined by two tailed, paired Student's t-test. * p<0.05; ** p<0.01; n.s. not significant.

Techniques: Cell Culture, Flow Cytometry, Concentration Assay, Two Tailed Test

5×10 5 lin − , c-kit + cells at a density of 5×10 5 cells/ml were cultured on plates that had been coated with 10 µg/ml of Fc or CD137-Fc protein or that were not coated (PBS, medium control). Where indicated, G-CSF was added daily at 10 ng/ml. The percentages (A) and absolute numbers (B) of CD11b + , Ly6G − and CD11b + , Ly6G + cells, and the percentages (C) and absolute numbers (D) of CD115 + , MPO − and CD115 − , MPO + cells were determined by flow cytometry on day 7. (A) and (C) are representatives of five and two experiments, respectively, (B) and (D) are depicted as means ± standard errors of five and two independent experiments, respectively. * p<0.05.

Journal: PLoS ONE

Article Title: Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

doi: 10.1371/journal.pone.0015565

Figure Lengend Snippet: 5×10 5 lin − , c-kit + cells at a density of 5×10 5 cells/ml were cultured on plates that had been coated with 10 µg/ml of Fc or CD137-Fc protein or that were not coated (PBS, medium control). Where indicated, G-CSF was added daily at 10 ng/ml. The percentages (A) and absolute numbers (B) of CD11b + , Ly6G − and CD11b + , Ly6G + cells, and the percentages (C) and absolute numbers (D) of CD115 + , MPO − and CD115 − , MPO + cells were determined by flow cytometry on day 7. (A) and (C) are representatives of five and two experiments, respectively, (B) and (D) are depicted as means ± standard errors of five and two independent experiments, respectively. * p<0.05.

Techniques: Cell Culture, Control, Flow Cytometry

(A) Lin − , c-kit + cells (boxed area of left panel) were sorted for CD34 + , CD16/32 high (GMP) and CD34 + , CD16/32 low (CMP) cells (right panel). (B) 5×10 4 CMP or GMP at a density of 2.5×10 5 cells/ml were cultured for 7 days on plates that had been coated with 10 µg/ml of Fc or CD137-Fc protein or that were not coated (PBS, medium control). Photographs were taken on day 7 at magnification of ×200. Scale bar = 50 µm. Please note that the small round particles are cell debris as evidenced by their small size and the holes in the membranes. (C) The percentages of CD11b + , Ly6G − and CD11b + , Ly6G + cells of (B) were determined by flow cytometry.

Journal: PLoS ONE

Article Title: Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

doi: 10.1371/journal.pone.0015565

Figure Lengend Snippet: (A) Lin − , c-kit + cells (boxed area of left panel) were sorted for CD34 + , CD16/32 high (GMP) and CD34 + , CD16/32 low (CMP) cells (right panel). (B) 5×10 4 CMP or GMP at a density of 2.5×10 5 cells/ml were cultured for 7 days on plates that had been coated with 10 µg/ml of Fc or CD137-Fc protein or that were not coated (PBS, medium control). Photographs were taken on day 7 at magnification of ×200. Scale bar = 50 µm. Please note that the small round particles are cell debris as evidenced by their small size and the holes in the membranes. (C) The percentages of CD11b + , Ly6G − and CD11b + , Ly6G + cells of (B) were determined by flow cytometry.

Techniques: Cell Culture, Control, Flow Cytometry